Composite

Part:BBa_K3763001:Design

Designed by: Liran Mao   Group: iGEM21_WHU-China   (2021-10-18)


Over-expression of FadR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This part is designed for better reducing expression leakage so it is closely related to BBa_K3763002 and BBa_K3763003.


Source

Designed by our team members on the basis of BBa_K817007, BBa_K817001 and https://www.ncbi.nlm.nih.gov/gene/948652, GenBank: X08087.1

References